Preliminary reports of parts of this work have been published (Raijman, L., Cheung, C-W., and Cohen, N. Given the rates of citrulline and urea synthesis which occur in vivo and the concentrations of ornithine present in the liver, our findings indicate that ornithine may contribute to the physiological regulation of urea synthesis.
within the physiological range, this amino acid is undetectable in the matrix. Lets load the filtered Cell Ranger h5 matrices downloaded from 10x. At external ornithine concentrations less than or equal to 1 mM, i.e. Figure 9.2: Label-centric dataset comparison can project cells from a new experiment. At rates of carbamyl phosphate synthesis of 10-50 nmol/min/mg, the rate of citrulline synthesis is controlled by external ornithine in the range 0.03-0.2 mM at greater than or equal to 0.2 mM ornithine, transport is not limiting for citrulline synthesis. The rate of ornithine transport can be greater than 80 nmol/min/mg. The channeling of ornithine suggests that some soluble enzymes are organized within the mitochondrial matrix. External ornithine is channeled between its transporter and ornithine transcarbamylase mitochondria preloaded with cold ornithine, then incubated with ornithine, produced citrulline of the same specific radioactivity as that of external ornithine, while matrix ornithine remained essentially unlabeled. In the presence of citrulline synthesis, we made the following observations.
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